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Dietary probiotic live yeast modulates antioxidant enzyme activities and gene expression of sea bass (Dicentrarchus labrax) larvae ArchiMer
Tovar-ramirez, D.; Mazurais, David; Gatesoupe, J. F.; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, J. L..
The main goal of this work was to determine the effect of dietary live yeast Debaryomyces hansenii on the enzymatic antioxidative status of sea bass Dicentrarchus labrax larvae. Growth, activity and expression of the main antioxidative enzymes: catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD), and heat shock protein (HSP70) were measured in sea bass larvae at 23 and 48 days after hatching. Larvae were fed on two microdiets: group one, fed microdiet containing live yeast and the control group fed microdiet without yeast. Heat shock protein 70 showed the same expression levels in both fish larvae fed yeast and the control diet. The group fed D. hansenii showed highest growth and lower activity and expression levels of GPX and SOD...
Tipo: Text Palavras-chave: Antioxidant enzymes; Probiotics; Debaryomyces hansenii; Dicentrarchus labrax.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11299/7881.pdf
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Domestication, sélection et comportement du bar: variabilité des aptitudes comportementales et de la tolérance au stress de groupes génétiquement distincts de bar, Dicentrarchus labrax ArchiMer
Millot, Sandie.
The culture of aquatic species has grown much more rapidly in recent years than that of terrestrial species. Consequently, aquatic livestock welfare is a growing concern for society. However, unlike the many studies of terrestrial animal welfare, few scientific data exist concerning fish welfare. It is generally recognized that the aquaculture environment exposes fish to many repeated stress factors which affect their health, growth, reproductive performance and feeding behaviour. Even if stress responses do not highlight all welfare disturbances, it is generally admitted that they reveal a poor welfare. Among the solutions investigated to reduce fish stress, domestication and selection appear to be the favoured approaches. However, selection has generally...
Tipo: Text Palavras-chave: Personality; Physiological status; Growth; Swimming behaviour; Feeding behaviour; Stress tolerance; Adaptation; Welfare; Chronic stress; Acute stress; Selection; Domestication; Dicentrarchus labrax; Personnalité; Statut physiologique; Croissance; Comportement natatoire; Comportement alimentaire; Tolérance au stress; Adaptation; Bien être; Stress chronique; Stress aigu; Sélection; Domestication; Dicentrarchus labrax.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-6272.pdf
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Early individual electronic identification of sea bass using RFID microtags: A first example of early phenotyping of sex-related growth ArchiMer
Ferrari, Sebastien; Chatain, Beatrice; Cousin, Xavier; Leguay, Didier; Vergnet, Alain; Vidal, Marie-odile; Vandeputte, Marc; Begout, Marie-laure.
Although individual electronic tagging using PIT (Passive Integrated Transponder) tags is well established, it is mainly used for fish > 60 mm in length. Since electronic tagging is an ideal identification method, we used RFID (Radio Frequency Identification) microtags (6 mm in length, 1 mm in diameter, 10 mg in mass) to characterize individual fish from the early stages of their development and throughout their lifecycles. We used sea bass, Dicentrarchus labrax, (105 day-old fish weighing between 100 and 1100 mg) and studied the effects of intra-coelomic tagging in half the population using different endpoints including survival and tag reading, growth over 6 months and swimming responses. Dead fish were counted daily, biometric data were collected at...
Tipo: Text Palavras-chave: Tagging effects; Swimming behavior; Sexual dimorphism; Dicentrarchus labrax.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28645/27072.pdf
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Effect of chronic exposure to ammonia on alterations of proteins and immunoglobulins in sea bass (Dicentrarchus labrax) serum ArchiMer
Coeurdacier, Jean-luc; Dutto, Gilbert.
The relationship between serum protein, immunoglobulin concentrations and protein molecular weight profile (PMWP) alterations of sea bass (131.3 +/- 4.3 g) reared in sea water with sublethal concentrations of ammonia was studied over two periods totalling 116 days. During the exposure period (62 days) the first group (group 1) lived in sea water with 0.204 mg.L-1 unionized ammonia nitrogen equivalent to 12 % of the lethal concentration for 50 % of a population exposed for 96 h to ammonia (96-h LC50), whilst the second group (group 2) lived in sea water with 0.340 mg.L-1 UIA-N equivalent to 20 % of the 96-h LC50 of ammonia. Then, the two groups were left for a recovery period (54 days) in the same water as the control group (group 0). The determination of...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Fish culture; Proteins; Immunoglobulin; Sublethal toxicity; Ammonia; Dicentrarchus labrax; Poisson d'élevage; Protéine; Immunoglobuline; Toxicité sublétale; Ammoniaque.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-858.pdf
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Effect of dietary water content on European sea bass (Dicentrarchus labrax) growth and disease resistance ArchiMer
Przybyla, Cyrille; Fievet, Julie; Callier, Myriam; Blancheton, Jean-paul.
The effect of dietary water content on Dicentrarchus labrax growth parameters and resistance to Vibrio anguillarum infection was investigated using commercial pellets with identical energy contents and different moisture levels. The first experiment hypothesis was that moisturizing pelleted ration can have an impact on Dicentrarchus labrax growth performance by the osmoregulation energy cost reduction. In a second time, the experiment explores the effect of water addition in pellets on the fish resistance to a disease. A specific device was built to uniformly moisturize dry pellets to different moisture levels, i.e. 8%, 20%, 40% and 60%. After an acclimation period and a 54-day rearing period, the control fish had grown from 72.7 +/- 17.9 g to 133.3 +/-...
Tipo: Text Palavras-chave: Fish aquaculture; Dietary moisture content; Feed pellets; Fish growth; Immunity; Dicentrarchus labrax; Vibrio anguillarum.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00248/35927/34446.pdf
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Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass ArchiMer
Masroor, Waliullah; Farcy, Emilie; Blondeau-bidet, Eva; Venn, Alexander; Tambutté, Eric; Lorin-nebel, Catherine.
The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl−-cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Ion transporter; Gill; MRNA expression; Thermal acclimation; Salinity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00514/62600/66958.pdf
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Effect of selection for fasting tolerance on feed intake, growth and feed efficiency in the European sea bass Dicentrarchus labrax ArchiMer
Daule, Sophie; Vandeputte, Marc; Vergnet, Alain; Guinand, Bruno; Grima, Laure; Chatain, Beatrice.
Feed efficiency is a major goal for aquaculture sustainability, and selecting fish to genetically enhance this trait would be highly valuable. However, no selective breeding program specifically targeted to feed efficiency exists for farmed fish, mostly because of the difficulty of measuring individual feed intake. However, a negative phenotypic correlation between feed efficiency and weight loss at fasting has been previously demonstrated in sea bass submitted to feed deprivation (FD). We mated sea bass parents selected for their high (FD+) or low (FD-) weight loss at fasting to produce FD+ and FD- progeny, which were reared in a single tank to avoid common environmental effects. At 8 months of age, 1200 of those fish were submitted to three alternating...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Selective breeding; Feed efficiency; Fasting tolerance.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/29985/28483.pdf
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Effects de le temperature et du regime alimentaire sur certains parametres de l'immunite cellulaire du loup de mer en elevage ArchiMer
Deschaux, P; Peres, G.
The data showed that antibody response and NK cells activity of a teleostean fish Dicentrarchus labrax (Loup de mer) were maximal when animals were bred at a temperature equal to 18 degree C. A proteinoprive diet enhanced T cytotoxic response and diminished NK cell activity. A fast of 15 days had no effect on these two cellular responses.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Antibodies; Starvation; Toxicity; Cells; Immunity; Diets; Nutrition; Temperature effects; Fish culture.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1248.pdf
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Effects of dietary tannin on growth, feed utilization and digestibility, and carcass composition in juvenile European seabass ( Dicentrarchus labrax L.) ArchiMer
Omnes, Marie-helene; Le Goasduff, Julien; Le Delliou, Herve; Le Bayon, Nicolas; Quazuguel, Patrick; Robin, Jean.
Plant-based products in fish diets are valuable protein alternatives to fishmeal for the aquafeed industry. Many plant feed ingredients contain polyphenolic compounds, including tannins, which can have beneficial or adverse effects. The tolerable threshold of ingested tannins is unknown for marine carnivorous fishes. We studied the effects of tannic acid (TA) supplementation to the diet of juvenile European seabass (Dicentrarchus labrax) by measuring growth, feed utilization and digestibility, and carcass composition. We randomly allocated groups of fish (initial mean body weight of 10.2 ± 0.7 g; n = 40 fish per tank) to 12 replicate cylindrical-conical tanks (three per treatment). The fish were assigned to one of four dietary treatments for five weeks:...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Polyphenolic tannin; Growth; Digestibility; Carcass composition.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00374/48478/48767.pdf
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Environment and immunomodulation : effects of the soluble fraction of Norwegian heavy fuel on the immune system of Sea bass, Dicentrarchus labrax ArchiMer
Bado-nilles, Anne; Quentin, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Auffret, Michel; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune...
Tipo: Text Palavras-chave: PaHs; Fish; Contamination; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3427.pdf
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Estimates of heritability and genotype-environment interactions for body weight in sea bass (Dicentrarchus labrax L.) raised under communal rearing conditions ArchiMer
Saillant, Eric; Dupont Nivet, Mathilde; Haffray, Pierrick; Chatain, Beatrice.
Genetic parameters for somatic growth rate in sea bass were estimated in two experiments where 27 (year-class 1997) to 30 (year-class 1998) families of sea bass (3 dams mated with 9-10 sires according to a full factorial design) were raised mixed in the same tanks starting shortly before hatching (48 h post fertilization). Trials were performed under two temperature (experiment 1, 1997: group high temperature (HT) and group low temperature (LT)) and two density regimes (experiment 2, 1998: group high density (HD) and group low density (LD)) with a subset of the mating design (18 families) being reproduced in the two experiments. All the fish were genotyped at 3-6 microsatellite loci which allowed them to be assigned to the individual breeders used in the...
Tipo: Text Palavras-chave: Genotype x environment interaction; Communal rearing; Microsatellites markers; Dicentrarchus labrax; Growth; Heritability.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1706.pdf
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Estimation et amélioration des performances zootechniques de l'élevage larvaire de Dicentrarchus labrax et de Sparus auratus ArchiMer
Chatain, Beatrice.
By gathering specific measurement criteria and an objective analysis of field methods, the main purpose of this work is to help estimate larval Sea Bass (Dicentrarchus labrax) and Sea Bream {Sparus auratus) stock performance raised under intensive conditions. As such, it is a methodology guide zootechnicians can use, in their daily quantitative and qualitative evaluation stock issues. The first part of this work covers defining the precision of current counting methods and establishing their limitations. The quantification methods analysed, as well as the new proposed methods, relate to the major phases of larval farming, from laying to postlarval stage. Three field methods commonly used in aquaculture to quantify egg population are therefore...
Tipo: Text Palavras-chave: Sparus auratus; Dicentrarchus labrax; Larval breeding; Zootechnical performance; Sparus auratus; Dicentrarchus labrax; Elevage larvaire; Performance zootechnique.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/these-1750.pdf
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Etude cytologique ultrastructurale des anomalies hepatiques du loup, de la daurade, et de l'anguille, induites par une alimentation artificielle ArchiMer
Bac, N; Biagianti, S; Brusle, J.
In the three species Dicentrarchus labrax, Sparus auratus , and Anguilla anguilla an artificial feeding (dry pellets) disturbs liver cells as revealed at ultrastructural level. In hepatocytes, anomalies consist of lipids storage (liposomes) considered as deviation in fatty acids metabolism. Moreover are observed cellular alterations (mitochondrial vacuolization, nuclear pycnosis) related to degenerative process. It is of great interest to understand wether liver modifications are just a reaction to an unbalanced feeding or a true trophic pathology.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Natural populations; Fish culture; Lipids; Fatty acids; Cytology; Ultrastructure; Cells; Diseases; Pellet feeds; Metabolism; Liver; Artificial feeding; Nutrition disorders.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1250.pdf
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Etude morphologique du loup Dicentrarchus labrax L. de la region de Sete ArchiMer
Barnabe, Gilbert.
Sampling of a population of D. Labrax was one with spear diving catches. Morphometric and meristic data were used to calculate a biometric index. This index showed that all the bass of the Sete area belong to Dicentrarchus labrax, even if they were black spotted on the upper part of the body. Morphology was slightly different according to sex, but sex determination was not always possible using morphometric data. This inshore population shows some morphological characteristics (branchiospine and lateral line scale number) different from the data collected on the same sp along the Maroc Atlantic coast by Gravier, 1961.
Tipo: Text Palavras-chave: Mediterranean sea; Biometry; Anatomy; Morphology; Dicentrarchus labrax.
Ano: 1973 URL: http://archimer.ifremer.fr/doc/1973/publication-2153.pdf
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Evaluation des performances et de la qualité de triploïdes de bar (Dicentrarchus labrax) ArchiMer
Falguiere, Jean-claude.
Tipo: Text Palavras-chave: Larvae; Triploïd; Dicentrarchus labrax.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/rapport-2077.pdf
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Experiences de reproduction artificielle de Loup Dicentrarchus labrax (Linne 1758). ArchiMer
Barnabe, G.; Tournamille, J.-c..
Mature eggs were expressed from captured female and fertilised artificially with slightly bloody sperm, in dry conditions or in a humid atmosphere, both at 13 degree C. The best incubation conditions proved to be 13 degree C, with streptomycin and penicillin. Another sample was kept at 18 degree C. Hatching occurred after 4 or 5 days, larvae showing positive phototropism. The system of renewal of water and artificial food is described. The embryonic reserve drop of lipid disappeared between day 14 and 16. Observations on larvae were like previous ones, but live larvae had larger mouths than had fixed specimens, and it was found that temperatures of 13 degree C or 18 degree C were suitable beyond the egg stage. A sudden change in temperature caused by a...
Tipo: Text Palavras-chave: Temperature; Sperm; Eggs; Dicentrarchus labrax; Artificial reproduction.
Ano: 1972 URL: http://archimer.ifremer.fr/doc/1972/publication-2063.pdf
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Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater ArchiMer
Siddique, Mohammad A. Momin; Butts, Ian A. E.; Linhart, Otomar; Macias, Adrian D.; Fauvel, Christian.
Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of...
Tipo: Text Palavras-chave: Egg longevity; Overripe eggs; Reproductive ecology; Mediterranean sea bass; Dicentrarchus labrax.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00279/39000/37580.pdf
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Fitness of early life stages in F1 interspecific hybrids between Dicentrarchus labrax and D. punctatus ArchiMer
Ky, Chin-long; Vergnet, Alain; Molinari, Nicolas; Fauvel, Christian; Bonhomme, Francois.
Inter- and intraspecific crossbreeding experiments were conducted to evaluate the aquaculture potential of hybrids in the genus Dicentrarchus, focusing on fertilisation and hatching success. The experimental design consisted of 24 controlled crosses in which individual and pooled fertilisations were made between wild D. labrax individuals (8 dams and 5 sires originating from West Mediterranean and Atlantic populations) and wild D. punctatus (6 sires). Three experiments were successively performed: (1) dams from the Mediterranean population, individually considered to have good egg quality, (2) dams from Mediterranean population, individually considered to have poor egg quality and (3) crossbreeding using pooled eggs from the Atlantic population. In each...
Tipo: Text Palavras-chave: Sea bass; Hybrid progeny; Offspring quality; Fitness; Dicentrarchus labrax; Dicentrarchus punctatus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18881/16451.pdf
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Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla) ArchiMer
Peruzzi, Stefano; Chatain, Beatrice; Menu, Bruno.
The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant...
Tipo: Text Palavras-chave: Anguilla anguilla; Liza ramada; Sparus aurata; Dicentrarchus labrax; Teleosts; DNA content.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-411.pdf
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Gene-centromere mapping in meiotic gynogenetic European seabass ArchiMer
Oral, Munevver; Colleter, Julie; Bekaert, Michhttps://w3.ifremer.fr/archimer-admin/author.jsp#ael; Taggart, John B.; Palaiokostas, Christos; Mcandrew, Brendan J.; Vandeputte, Marc; Chatain, Beatrice; Kuhl, Heiner; Reinhardt, Richard; Peruzzi, Stefano; Penman, David J..
Background Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results From a single meiotic gynogenetic family consisting of 79 progeny, 42...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Meiotic gynogenesis; Isogenic lines; DdRAD seq; Genetic map; Gene-Centromere map; Aquaculture.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49901/50462.pdf
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